ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of hepatitis delta virus (HDV) marker among hepatitis B virus (HBV) infected patients and to reveal its clinical significance.</p><p><b>METHOD</b>To collect the clinical data and sera samples of HBV infected patients and to detect HDAg, Anti-HDV as well as HBV infection markers by means of enzyme-linked immunosorbnent assay. These data combined with clinical diagnostic results and biochemical index were then analyzed.</p><p><b>RESULT</b>462 samples of HBV infected patients were collected including 210 HBV carriers without symptom, 175 chronic HBV infections, 35 acute HBV infections and 42 liver fibrosis. The HDV infection rate was 4.8% overall. The highest infection rate of 9.5% was found in the group of liver fibrosis whereas the lower rate of 6.9% was found in HBV chronic carriers. HDV infection rate was 7.8% among the population of 40-60 years old, obviously higher than any other age groups.</p><p><b>CONCLUSION</b>HDV infection was significantly higher in the chronic HBV patients and liver fibrosis patients. Because HDV infection was highly associated with the progress of liver disease, we suggest the screen of HDV markers among hepatitis patients and discriminate whether the patient was co-infected with HDV.</p>
Subject(s)
Adolescent , Adult , Aged , Child , Female , Humans , Male , Middle Aged , Young Adult , Biomarkers , Blood , Coinfection , Blood , Diagnosis , Allergy and Immunology , Virology , Hepatitis Antibodies , Allergy and Immunology , Hepatitis B , Blood , Diagnosis , Allergy and Immunology , Virology , Hepatitis B Antigens , Blood , Hepatitis B virus , Allergy and Immunology , Physiology , Hepatitis D , Blood , Diagnosis , Allergy and Immunology , Virology , Hepatitis Delta Virus , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>This study attempted to explore the value of combining serum hepatic fibrosis-related markers and ultrasound parameters together on diagnosis of hepatic fibrosis.</p><p><b>METHODS</b>Six serum markers and 8 ultrasound parameters were measured from 100 patients with chronic hepatitis B or cirrhosis. The results of the serum hepatic fibrosis-related markers and ultrasound in disease group were analyzed and compared with the findings of hepatic pathology.</p><p><b>RESULTS</b>By filtrating,the group of platelet derived growth factor-BB (PDGF-BB) plus hyaluronic acid (HA) plus echo characteristics of liver parenchyma (LPEC) plus length of spleen (SL) had the highest Se and Spe, which were 90.7% and 85.4% respectively.</p><p><b>CONCLUSION</b>The advantageous combination of serum markers and ultrasound parameters can significantly improve Se and Spe, which is superior to any single serum index or ultrasound parameter. And it was a better non-invasive method for diagnosing hepatic fibrosis.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Alanine Transaminase , Blood , Biomarkers , Blood , Collagen Type III , Blood , Hepatitis B, Chronic , Blood , Diagnosis , Diagnostic Imaging , Hyaluronic Acid , Blood , Liver Cirrhosis , Blood , Diagnosis , Diagnostic Imaging , Platelet-Derived Growth Factor , Proto-Oncogene Proteins c-sis , Reproducibility of Results , Sensitivity and Specificity , Tissue Inhibitor of Metalloproteinase-1 , Blood , Transforming Growth Factor beta , Blood , Ultrasonography, Doppler, Color , MethodsABSTRACT
Objective To assess the clinical significance of antibodies against cyclic citrullinated peptide(anti - CCP) in a cohort of patients with juvenile idiopathic arthritis (JIA). Methods The anti - CCP was tested by an enzyme linked immunosorbent assay (ELISA) in serum samples from 22 patients with JIA(9 boys, 13 girls), with a mean age of 9.1 years(range 1.1 - 16). As control groups, anti - CCP were also tested in sera of 20 healthy children, and 30 adult patients with rheumatoid arthritis(RA). Results Positive anti - CCP were found in sera of 2 patients with JIA(9.1 %), both with lower titre comparing with those of adult patients with anti - CCP positive. In the control groups, none of healthy children was positive for anti - CCP, but 16/30(53. 3%) adults with RA showed anti - CCP positivity. Conclusion Anti - CCP can be detected in children with JIA, but are less frequently present than in adults with RA, so anti - CCP has limited diagnostic value to JIA.